Organoids focus

The OT news section is dedicated to the latest research about and featuring organoids.

Selected News

RSS feeds on organoids

RSS Pubmed

  • iPSC-derived Airway Epithelial Cells: Progress, Promise, and Challenges 3 octobre 2022
    Induced pluripotent stem cells (iPSCs) generated from somatic cell sources are pluripotent and capable of indefinite expansion in vitro. They provide an unlimited source of cells that can be differentiated into lung progenitor cells for the potential clinical use in pulmonary regenerative medicine. This review gives a comprehensive overview on recent progress toward the use […]
    Fenggang Yu
  • Interleukin-22 regulates neutrophil recruitment in ulcerative colitis and is associated with resistance to ustekinumab therapy 3 octobre 2022
    The function of interleukin-22 (IL-22) in intestinal barrier homeostasis remains controversial. Here, we map the transcriptional landscape regulated by IL-22 in human colonic epithelial organoids and evaluate the biological, functional and clinical significance of the IL-22 mediated pathways in ulcerative colitis (UC). We show that IL-22 regulated pro-inflammatory pathways are involved in microbial recognition, cancer […]
    Polychronis Pavlidis
  • Ensembles of endothelial and mural cells promote angiogenesis in prenatal human brain 30 septembre 2022
    Interactions between angiogenesis and neurogenesis regulate embryonic brain development. However, a comprehensive understanding of the stages of vascular cell maturation is lacking, especially in the prenatal human brain. Using fluorescence-activated cell sorting, single-cell transcriptomics, and histological and ultrastructural analyses, we show that an ensemble of endothelial and mural cell subtypes tile the brain vasculature during […]
    Elizabeth E Crouch
  • Proper acquisition of cell class identity in organoids allows definition of fate specification programs of the human cerebral cortex 30 septembre 2022
    Realizing the full utility of brain organoids to study human development requires understanding whether organoids precisely replicate endogenous cellular and molecular events, particularly since acquisition of cell identity in organoids can be impaired by abnormal metabolic states. We present a comprehensive single-cell transcriptomic, epigenetic, and spatial atlas of human cortical organoid development, comprising over 610,000 […]
    Ana Uzquiano
  • Multispectral confocal 3D imaging of intact healthy and tumor tissue using mLSR-3D 30 septembre 2022
    Revealing the 3D composition of intact tissue specimens is essential for understanding cell and organ biology in health and disease. State-of-the-art 3D microscopy techniques aim to capture tissue volumes on an ever-increasing scale, while also retaining sufficient resolution for single-cell analysis. Furthermore, spatial profiling through multi-marker imaging is fast developing, providing more context and better […]
    Ravian L van Ineveld
  • The NALCN channel regulates metastasis and nonmalignant cell dissemination 29 septembre 2022
    We identify the sodium leak channel non-selective protein (NALCN) as a key regulator of cancer metastasis and nonmalignant cell dissemination. Among 10,022 human cancers, NALCN loss-of-function mutations were enriched in gastric and colorectal cancers. Deletion of Nalcn from gastric, intestinal or pancreatic adenocarcinomas in mice did not alter tumor incidence, but markedly increased the number […]
    Eric P Rahrmann
  • Cytokine responsive networks in human colonic epithelial organoids unveil a molecular classification of inflammatory bowel disease 28 septembre 2022
    Interactions between the epithelium and the immune system are critical in the pathogenesis of inflammatory bowel disease (IBD). In this study, we mapped the transcriptional landscape of human colonic epithelial organoids in response to different cytokines responsible for mediating canonical mucosal immune responses. By profiling the transcriptome of human colonic organoids treated with the canonical […]
    Polychronis Pavlidis
  • A nomenclature consensus for nervous system organoids and assembloids 28 septembre 2022
    Self-organizing three-dimensional cellular models derived from human pluripotent stem cells or primary tissue have great potential to provide insights into how the human nervous system develops, what makes it unique and how disorders of the nervous system arise, progress and could be treated. Here, to facilitate progress and improve communication with the scientific community and […]
    Sergiu P Pașca
  • c-Myc plays a key role in IFN-γ-induced persistence of <em>Chlamydia trachomatis</em> 26 septembre 2022
    Chlamydia trachomatis (Ctr) can persist over extended times within their host cell and thereby establish chronic infections. One of the major inducers of chlamydial persistence is interferon-gamma (IFN-γ) released by immune cells as a mechanism of immune defence. IFN-γ activates the catabolic depletion of L-tryptophan (Trp) via indoleamine-2,3-dioxygenase (IDO), resulting in persistent Ctr. Here, we […]
    Nadine Vollmuth
  • A microfluidic platform to investigate the role of mechanical constraints on tissue reorganization 26 septembre 2022
    Mechanical constraints have a high impact on development processes, and there is a need for new tools to investigate the role of mechanosensitive pathways in tissue reorganization during development. We present here experiments where embryonic cell aggregates are aspired through constrictions in microfluidic channels, generating highly heterogeneous flows and high cell deformations that can be […]
    S Tlili